polymorphonuclear neutrophils pmns  (Bio-Rad)

Journal: Pathogens

Article Title: Effect of Female Sex Hormones on the Immune Response against Chlamydia abortus and on Protection Conferred by an Inactivated Experimental Vaccine in a Mouse Model

doi: 10.3390/pathogens11010093

Figure Lengend Snippet: Characterization of the inflammatory infiltrate in the liver. Representative images of the hepatic lesions caused by C. abortus infection at day 8 pi. in non-treated infected control group (Control), oestradiol (E2) and progesterone (P4) treated mice. ( A ) Labelling with monoclonal antibodies against macrophages, ( B ) iNOS, ( C ) polymorphonuclear neutrophils (PMN), and ( D ) T lymphocytes (CD3).

Article Snippet: Additionally, characterisation of the inflammatory infiltrate was carried out in Zinc-fixed samples, using monoclonal antibodies against F4/80 for macrophages (Caltag Medsystem, Buckingham, UK), iNOS (Upstate Biotechnology, Lake Placid, NY, USA) that can be present in activated macrophages and other iNOS-producing cells, reviewd in [ ], CD3 for T lymphocytes (Dako-Agilent, Santa Clara, CA, USA), and NIMP-R14 for polymorphonuclear neutrophils (PMN) (Santa Cruz Biotechnology, Dallas, TX, USA), using Avidin-Biotin-Peroxidase Complex (ABC) (as described in [ ]).

Techniques: Infection, Control, Bioprocessing

Journal: Pathogens

Article Title: Effect of Female Sex Hormones on the Immune Response against Chlamydia abortus and on Protection Conferred by an Inactivated Experimental Vaccine in a Mouse Model

doi: 10.3390/pathogens11010093

Figure Lengend Snippet: Characterization of the inflammatory infiltrate at different times of infection in non-treated infected control group (Control), oestradiol (E2) and progesterone (P4) treated mice, using monoclonal antibodies against macrophages, detection of iNOS-producing cells, polymorphonuclear neutrophils (PMN) and T lymphocytes (CD3). Quantification of each cell type was calculated as percentage of immunolabelled area. Results from ten different fields per mouse are shown. Significant differences between treatments and different days of infection were estimated by one-way-ANOVA. A significant increase was found in the recruitment of macrophages between 4 and 8 days pi. for all groups and the effect of E2 was significant on day 8 pi. The effect of E2 treatment was also significant in the recruitment of PMNs at 8 days pi. (* p < 0.05, ** p < 0.01).

Article Snippet: Additionally, characterisation of the inflammatory infiltrate was carried out in Zinc-fixed samples, using monoclonal antibodies against F4/80 for macrophages (Caltag Medsystem, Buckingham, UK), iNOS (Upstate Biotechnology, Lake Placid, NY, USA) that can be present in activated macrophages and other iNOS-producing cells, reviewd in [ ], CD3 for T lymphocytes (Dako-Agilent, Santa Clara, CA, USA), and NIMP-R14 for polymorphonuclear neutrophils (PMN) (Santa Cruz Biotechnology, Dallas, TX, USA), using Avidin-Biotin-Peroxidase Complex (ABC) (as described in [ ]).

Techniques: Infection, Control, Bioprocessing

Journal: Pathogens

Article Title: Effect of Female Sex Hormones on the Immune Response against Chlamydia abortus and on Protection Conferred by an Inactivated Experimental Vaccine in a Mouse Model

doi: 10.3390/pathogens11010093

Figure Lengend Snippet: Characterisation of the inflammatory infiltrate in non-treated infected control group (Control), oestradiol (E2) and progesterone (P4) treated mice in non vaccinated (NV) and vaccinated mice (V). Monoclonal antibodies against macrophages, detection of iNOS from iNOS-producing cells, polymorphonuclear neutrophils (PMN) and T lymphocytes (CD3) were used. Quantification of each cell type was calculated as percentage of immunolabelled area. Results from ten different fields per mouse are shown. Significant differences between treatments and vaccination were estimated by one-way-ANOVA. iNOS labelling was significantly lower in all vaccinated mice than non-vaccinated groups. E2 treatment had a significant effect in the recruitment of T lymphocytes with higher percentage of positive area than Control and P4 groups (* p < 0.05).

Article Snippet: Additionally, characterisation of the inflammatory infiltrate was carried out in Zinc-fixed samples, using monoclonal antibodies against F4/80 for macrophages (Caltag Medsystem, Buckingham, UK), iNOS (Upstate Biotechnology, Lake Placid, NY, USA) that can be present in activated macrophages and other iNOS-producing cells, reviewd in [ ], CD3 for T lymphocytes (Dako-Agilent, Santa Clara, CA, USA), and NIMP-R14 for polymorphonuclear neutrophils (PMN) (Santa Cruz Biotechnology, Dallas, TX, USA), using Avidin-Biotin-Peroxidase Complex (ABC) (as described in [ ]).

Techniques: Infection, Control, Bioprocessing